Multiplex SNP detection in multiple codons for accurate drug therapy.
نویسندگان
چکیده
Multiplex SNP discrimination in the multiple codons of the genomic DNA is demonstrated by applying controller DNA technology (CDT) to MDR-TB 9G DNAChips. CDT allowed the efficient detection of 20 SNPs in five codons of the genomic DNA in 40 min. CDT could distinguish SNP targets to as low as 1 copy of the genomic DNA. 100% agreement with the sequencing analysis of clinical samples highlighted the clinical applicability of MDR-TB 9G DNAChips.
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ورودعنوان ژورنال:
- Chemical communications
دوره 50 93 شماره
صفحات -
تاریخ انتشار 2014